Low-calorie sweeteners have been the subject of extensive scientific research looking at their effect on a variety of health conditions. Low-calorie sweeteners have been consumed in food and beverage products, as well as in tabletop form, for many years. The approved low-calorie sweeteners and those that are generally recognized as safe (GRAS) have been reviewed and determined to be safe for human consumption by the Food and Drug Administration (FDA). Low-calorie sweeteners provide consumers with a broader selection of low-calorie, palatable foods, and offer a sweet alternative for people with diabetes, who must monitor their carbohydrate and sugar intakes carefully. Low-calorie sweeteners can also play a role in a weight management plan that incorporates healthful food choices and regular exercise. With conflicting information causing confusion and the perpetuation of myths and misperceptions, the following science-based resources set the record straight on low-calorie sweetener safety and use.